Shigella bacteria entry in a cell
Jost Enninga establishes novel tools that yield information on the molecular details of bacterial pathogen infection in the context of single living cells with unprecedented spatiotemporal resolution.
Pathogens have been evolving with their hosts for millions of years adopting tailored strategies to propagate within the host body and evading the host immune response. It is crucial to understand the underlying strategies employed by pathogens on the cellular and molecular level in order to develop novel weapons against them. On the cellular level, host-pathogen interactions are generally very dynamic and tightly regulated. Therefore, it has been difficult to establish methodologies that can capture this cross-talk. To overcome these hurdles, Jost Enninga and his team have been developing novel approaches to monitor the dynamic host-pathogen interactions.
Particularly, we have taken advantage of methodologies based on the principle of fluorescence, which we use and/or develop to obtain information on molecular processes in single living cells. We will address the following key questions in the field of cellular microbiology :
1/ how do entero-pathogenic bacteria invade cells
2/ how do the latter sense the incoming pathogens
Globally, using their cell-based assays, Jost Enninga and his team envision that they will obtain a precise idea of how bacterial pathogens undermine host cells in their favour, and how they escape the host immunity system. This will identify novel drug targets, and their assays can also be used to evaluate potential drugs. Finally, they will also be able to reveal fundamental cell biological mechanisms using the impact of pathogens on the host cells.
• 2003 Ph. D. in Cell Biology, The Rockefeller University, New York, USA, Guenter Blobel's lab, Transport of biological macromolecules between cellular compartments
• 2004-2008 Post-doctoral fellow, The Pasteur Institute, Paris, France, Phillipe Sansonetti and Guy Tran Van Nhieu, Secretion Type III effectors from Gram negative bacteria
• 2008 Appointed Group leader at the Pasteur Institute, Paris, France
• Starting Grant of the European Research Council (ERC), 2010
• Robert Koch Post-doctoral award, 2006
Aug 2017, Methods
Oct 2014, Cell Host Microbe
Feb 2012, PLoS Pathog
The “when and wereabouts” of injected pathogen effectors
Apr 2010, Nat Methods
Mar 2010, PLoS Genetics