"Human colonosphere or “mini-gut” from in vitro from patient’s Lieberkühn’s crypts."

Copyrights : ©E D’Aldebert / N. Vergnolle / Le Cercle FSER, CC-BY-NC-SA

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Image caption :

“Shadowed projection” (Imaris software) of half a human colonosphere composed of a single layer of epithelial cells delineating a central lumen. Nuclei are represented in green, actin in cyan and the proliferation marker Ki67 in red. Image acquired under a SP8 Leica confocal microscope.

The technical:

The colonosphere culture is novel technique allowing to generate human intestinal epithelium in 3D while maintaining activity regionalization (proliferation, differentiation and apoptosis) of cellular types (immature and differentiated cells).