Senior group leader, Research Director (DR2)
Curie Institute, Paris, France Inserm, France
Mouse embryos at different developmental stages
Déborah Bourc’his is focused on understanding the role of epigenetics in mammalian reproduction. Her efforts with her team are mostly centered on DNA methylation, and they are addressing the following questions: How are DNA methylation patterns shaped? How do they impact germ cell identity and integrity? How much gametic DNA methylation is transmitted to the progeny and how does it influence phenotypes across generations?
By standing at the crossroads of generations, germ cells ensure species continuity. At the time of fertilization, the oocyte and spermatozoon carry the genetic material but also non-genetically encoded, epigenetic information. Gametic epigenetic modifications have immediate effects on gametic production and fertility. They also have long-term consequences on somatic phenotypes when transmitted to the progeny.
Realizations / Projects
Déborah Bourc’his and her team have been focused on understanding the role of epigenetics in mammalian reproduction. Our efforts are mostly centered on DNA methylation, and we are addressing the following questions: How are DNA methylation patterns shaped? How do they impact on germ cell identity and integrity? How much gametic DNA methylation is transmitted to the progeny and how does this influence phenotypes across generations?
Studying the methylome of germ cells touches on several crucial aspects of mammalian biology such as transposon control, genomic imprinting and cell identity. Studying DNA methylation in the window of conception can moreover provide mechanistic insights into the spatio-temporal control of genomic methylation specificity, which include components of histone modification and (small and long) RNA-directed pathways.
Our projects can be subdivided into three interconnected themes: 1) Trans and cis determinants of de novo DNA methylation,
2) DNA methylation and transposon control, and
3) DNA methylation and genomic imprinting. Our approach is mainly fundamental, using the mouse as a mammalian model, and involves a combination of genetics, cellular and developmental biology, with large-scale genomic and biochemical strategies. We are also extending our research to humans, in the hope of uncovering new causes of impaired or malignant gametogenesis.
Correct DNA methylation patterns are paramount for the generation of functional gametes capable of forming viable and healthy offspring, but also for the regulation of pluripotency states and the maintenance of genome architecture and function in somatic cells. Our work therefore not only impacts on the field of reproduction and development, but also on stem cell biology and cancer.
• 2000, PhD in Natural Sciences, Necker Hospital, France. Evani Viegas-Péguignot's lab, Genetic and embryonic origins of the DNA methylation defect observed in ICF patients.
• 2001-2005 Postdoctoral fellow at Columbia University, USA. Timothy Bestor’s Lab, Dnmt3L roles in genomic imprinting establishment and transposon control during mammalian gametogenesis
• 2005-2008 Research scientist at Paris 7 University, France, Genomic and functional characterization of genomic imprinting
• 2009 Appointed Team Leader, Institut Curie, Paris, France
• Liliane Bettencourt Prize for Life Sciences, Fondation Bettencourt Schueller, 2017
• Suffrage Award-Women in Science, MRC London, 2016
• Elected EMBO member, 2014
• Consolidator ERC (European Research Council) Grant 2013
• Jayle Prize, French Academy of Science, 2013
• European Young Investigator Award, European Science Foundation, 2006
Transient transcription in the early embryo sets an epigenetic state that programs post-natal growth.
Jan 2017, Nature Genetics
MORC1 represses transposable elements in the mouse male germline
Déc 2014, Nat Commun.
The Gpr1/Zdbf2 locus provides new paradigms for transient and dynamic genomic imprinting in mammals
March 2014, Genes and Dev